Construction of Human Gene Libraries from Small Amounts cf Peripheral Blood: Analysis of β-Like Globin Genes
M Poncz, D Solowiejczyk, B Harpel, Y Mory… - …, 1982 - Taylor & Francis
M Poncz, D Solowiejczyk, B Harpel, Y Mory, E Schwartz, S Surrey
Hemoglobin, 1982•Taylor & FrancisWe describe a rapid procedure for constructing cloned human genomic libraries from small
amounts of peripheral blood. High molecular weight DNA is isolated from 5–20 ml
peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using
bacteriophage Charon 4A and a suitable E. coli host. Using this approach we have isolated
and characterized several non-a globin clones from a Kurdish Jew with homozygous β
thalassemia. The ability to isolate suitable amounts of high molecular weight DNA from …
amounts of peripheral blood. High molecular weight DNA is isolated from 5–20 ml
peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using
bacteriophage Charon 4A and a suitable E. coli host. Using this approach we have isolated
and characterized several non-a globin clones from a Kurdish Jew with homozygous β
thalassemia. The ability to isolate suitable amounts of high molecular weight DNA from …
We describe a rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood. High molecular weight DNA is isolated from 5–20 ml peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using bacteriophage Charon 4A and a suitable E.coli host. Using this approach we have isolated and characterized several non-a globin clones from a Kurdish Jew with homozygous β thalassemia. The ability to isolate suitable amounts of high molecular weight DNA from peripheral blood provides a relatively simple means of constructing human gene libraries representing a variety of hemoglobin disorders.
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