Background Western blotting is used to measure protein expression in cells and tissues.
Appropriate interpretation of resulting data is contingent upon antibody validation.
Objectives We assessed several commercial anti‐human and anti‐mouse tissue factor (TF)
antibodies for their ability to detect TF by western blotting. Material and Methods We used
human pancreatic cancer cell lines expressing different levels of TF and a mouse pancreatic
cancer cell line expressing TF with a matched knockout derivative. Results Human and …