Analysis of circular RNAs using the web tool circinteractome
AC Panda, DB Dudekula, K Abdelmohsen… - Circular RNAs: methods …, 2018 - Springer
Circular RNAs: methods and protocols, 2018•Springer
Circular RNAs (circRNAs) are generated through nonlinear back splicing, during which the
5′ and 3′ ends are covalently joined. Consequently, the lack of free ends makes them
very stable compared to their counterpart linear RNAs. By selectively interacting with
microRNAs and RNA-binding proteins (RBPs), circRNAs have been shown to influence
gene expression programs. We designed a web tool, CircInteractome, in order to (1) explore
potential interactions of circRNAs with RBPs,(2) design specific divergent primers to detect …
5′ and 3′ ends are covalently joined. Consequently, the lack of free ends makes them
very stable compared to their counterpart linear RNAs. By selectively interacting with
microRNAs and RNA-binding proteins (RBPs), circRNAs have been shown to influence
gene expression programs. We designed a web tool, CircInteractome, in order to (1) explore
potential interactions of circRNAs with RBPs,(2) design specific divergent primers to detect …
Abstract
Circular RNAs (circRNAs) are generated through nonlinear back splicing, during which the 5′ and 3′ ends are covalently joined. Consequently, the lack of free ends makes them very stable compared to their counterpart linear RNAs. By selectively interacting with microRNAs and RNA-binding proteins (RBPs), circRNAs have been shown to influence gene expression programs. We designed a web tool, CircInteractome, in order to (1) explore potential interactions of circRNAs with RBPs, (2) design specific divergent primers to detect circRNAs, (3) study tissue- and cell-specific circRNAs, (4) identify gene-specific circRNAs, (5) explore potential miRNAs interacting with circRNAs, and (6) design specific siRNAs to silence circRNAs. Here, we review the CircInteractome tool and explain recent updates to the site. The database is freely accessible at http://circinteractome.nia.nih.gov .
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